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Addgene

GFAP-HSV-tk (pTGB008)
(Plasmid #24703)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24703 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    GFAP-lacZ
  • Backbone size w/o insert (bp) 18000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HSV-tk
  • Alt name
    thymidine kinase from herpes simplex virus (HSV-Tk)
  • Species
    HSV
  • Promoter GFAP (glial fibrillary acidic protein)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

All unidentified sequence is mouse genomic DNA encompassing the mouse GFAP (glial fibrillary acidic protein) gene.

HSV-TK + SV40 polyA cloned into NotI/SalI sites of GFAP-lacZ plasmid as described in Bush et al., (1998) Cell 93:189-201. Details of this promoter cassette (clone 445) can be found in Johnson et al (1995) Glia 13:174-184.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFAP-HSV-tk (pTGB008) was a gift from Michael Sofroniew (Addgene plasmid # 24703)

  • For your References section:

    Fulminant jejuno-ileitis following ablation of enteric glia in adult transgenic mice. Bush TG, Savidge TC, Freeman TC, Cox HJ, Campbell EA, Mucke L, Johnson MH, Sofroniew MV. Cell. 1998 Apr 17. 93(2):189-201. 10.1016/S0092-8674(00)81571-8 PubMed 9568712
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