pGHNC5
(Plasmid #24701)

• Depositing Lab: George Allen
• Publication: Allen et al Plant Cell. 1996 May . 8(5):899-913.

Backbone

• Vector backbone: pKS II (Bluescript II KS+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2961
• Vector type: E. coli phagemid

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH 5 alpha overnight 37C, 225 rpm
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Direct Repeats of Tobacco Rb7-6 MARs
• Alt name: Rb7-6
• Alt name: Rb7 MAR
• Alt name: Rb7 SAR
• Species: Nicotiana tobacum
• Insert Size (bp): 2336
• GenBank ID: U67919

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI, ApaI, XhoI, SalI (HincII), AccI, ClaI/Sca (destroyed during cloning)
• 3′ cloning site: SmaI/Cla, BamHI, SpeI, XbaI, NotI (EagI), SacII, BstXI, SacI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pGH NC5 contains direct repeats of the tobacco Rb7 Matrix Attachment Region. The 5' and 3' sites mentioned earlier flank the repeat. However, at the 5'end of the first repeat is an MCS which contains SalI, ClaI, HindIII, EcoRV, PstI, SmaI, BamH1, SpeI. This is followed by the second Rb7 MAR.