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Addgene

pRB7-6
(Plasmid #24700)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24700 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKS II (Bluescript II KS+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Vector type
    E. coli phagemid

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH 5 alpha overnight 37C, 225 rpm
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tobacco Rb7 MAR (SAR)
  • Alt name
    RB7-6 Sc/Cl
  • Alt name
    pRB7-6
  • Alt name
    NC7-6
  • Species
    Nicotiana tabacum
  • Insert Size (bp)
    1168
  • GenBank ID
    U67919

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI, ApaI, XhoI, SalI (HincII), AccI, ClaI/Sca (destroyed during cloning)
  • 3′ cloning site SmaI/Cla, BamHI, SpeI, XbaI, NotI (EagI), SacII, BstXI, SacI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pRB7-6 contains the tobacco Rb7 Matrix Attachment Region (MAR).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRB7-6 was a gift from George Allen (Addgene plasmid # 24700 ; http://n2t.net/addgene:24700 ; RRID:Addgene_24700)

  • For your References section:

    High-level transgene expression in plant cells: effects of a strong scaffold attachment region from tobacco. Allen GC, Hall G, Michalowski S, Newman W, Spiker S, Weissinger AK, Thompson WF. Plant Cell. 1996 May . 8(5):899-913. 10.2307/3870291 PubMed 8672887
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