pCS2 xenopus Frzb-1
(Plasmid
#24694)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24694 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2
- Backbone size w/o insert (bp) 4100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFrzb-1
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SpeciesX. laevis (frog)
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Entrez Genefrzb-1 (a.k.a. frzb, Sfrp3, frezzled)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For antisense linearize with HindIII, transcribe with T7
For sense linearize with NotI, transcribe with SP6
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2 xenopus Frzb-1 was a gift from Edward De Robertis (Addgene plasmid # 24694 ; http://n2t.net/addgene:24694 ; RRID:Addgene_24694) -
For your References section:
A direct screen for secreted proteins in Xenopus embryos identifies distinct activities for the Wnt antagonists Crescent and Frzb-1. Pera EM, De Robertis EM. Mech Dev. 2000 Sep . 96(2):183-95. 10.1016/S0925-4773(00)00394-4 PubMed 10960783