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Addgene

G1A
(Plasmid #24690)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24690 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    puc18
  • Backbone manufacturer
    GenScript
  • Backbone size w/o insert (bp) 2686
  • Vector type
    plasmid cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human XIST
  • Alt name
    XIST
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    9400
  • Mutation
    Genomic insert. Includes introns 5-8.
  • GenBank ID
    NG_016172
  • Entrez Gene
    XIST (a.k.a. DXS1089, DXS399E, LINC00001, NCRNA00001, SXI1, swd66)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BgIII (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Huntinton Willard (Duke University).
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    G1A was a gift from Jeanne Lawrence (Addgene plasmid # 24690 ; http://n2t.net/addgene:24690 ; RRID:Addgene_24690)
  • For your References section:

    XIST RNA paints the inactive X chromosome at interphase: evidence for a novel RNA involved in nuclear/chromosome structure. Clemson CM, McNeil JA, Willard HF, Lawrence JB. J Cell Biol. 1996 Feb . 132(3):259-75. 10.1083/jcb.132.3.259 PubMed 8636206