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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24690 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepuc18
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Backbone manufacturerGenScript
- Backbone size w/o insert (bp) 2686
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Vector typeplasmid cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehuman XIST
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Alt nameXIST
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SpeciesH. sapiens (human)
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Insert Size (bp)9400
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MutationGenomic insert. Includes introns 5-8.
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GenBank IDNG_016172
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Entrez GeneXIST (a.k.a. DXS1089, DXS399E, LINC00001, NCRNA00001, SXI1, swd66)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BgIII (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byHuntinton Willard (Duke University).
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
G1A was a gift from Jeanne Lawrence (Addgene plasmid # 24690 ; http://n2t.net/addgene:24690 ; RRID:Addgene_24690) -
For your References section:
XIST RNA paints the inactive X chromosome at interphase: evidence for a novel RNA involved in nuclear/chromosome structure. Clemson CM, McNeil JA, Willard HF, Lawrence JB. J Cell Biol. 1996 Feb . 132(3):259-75. 10.1083/jcb.132.3.259 PubMed 8636206