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Addgene

pCS2 xIGF2
(Plasmid #24689)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24689 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Insulin-like growth factor 2
  • Alt name
    IGF2
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    478
  • Entrez Gene
    igf2-b (a.k.a. igf2, IGF-II, insigf, pp9974, MGC80113)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For sense linearize with NotI, transcribe with SP6.
For antisense, linearize with EcoRI, transcribe with T7.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2 xIGF2 was a gift from Edward De Robertis (Addgene plasmid # 24689 ; http://n2t.net/addgene:24689 ; RRID:Addgene_24689)

  • For your References section:

    Isthmin is a novel secreted protein expressed as part of the Fgf-8 synexpression group in the Xenopus midbrain-hindbrain organizer. Pera EM, Kim JI, Martinez SL, Brechner M, Li SY, Wessely O, De Robertis EM. Mech Dev. 2002 Aug . 116(1-2):169-72. 10.1016/S0925-4773(02)00123-5 PubMed 12128218
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