pCS2 xenopus sizzled
(Plasmid
#24684)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24684 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2
- Backbone size w/o insert (bp) 4100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSizzled
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SpeciesX. laevis (frog)
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Insert Size (bp)1200
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Entrez Geneszl (a.k.a. xszl)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Best in situ probe.
For anitsense: linearize with NotI
Transcribe with SP6
For mRNA injections use 200 pg to dorsalize.
E248D does not affect function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2 xenopus sizzled was a gift from Edward De Robertis (Addgene plasmid # 24684 ; http://n2t.net/addgene:24684 ; RRID:Addgene_24684) -
For your References section:
Embryonic dorsal-ventral signaling: secreted frizzled-related proteins as inhibitors of tolloid proteinases. Lee HX, Ambrosio AL, Reversade B, De Robertis EM. Cell. 2006 Jan 13. 124(1):147-59. 10.1016/j.cell.2005.12.018 PubMed 16413488