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Addgene

pCS2 xenopus chordin myc
(Plasmid #24682)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24682 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Chordin
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    3797
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Chordin expression vector, tagged. Insert of pSP35 Chd-Myc (Chd coding region). Insert cut from pSP35Chd-Myc (from a dcm-, dam- bacterial strain, as Xba I is there in a methylation consensus) with EcoRI and XbaI and subcloned in the same sites of pCS2.

For sense RNA injection: linearize with NotI
Transcribe with SP6

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2 xenopus chordin myc was a gift from Edward De Robertis (Addgene plasmid # 24682 ; http://n2t.net/addgene:24682 ; RRID:Addgene_24682)

  • For your References section:

    Dorsoventral patterning in Xenopus: inhibition of ventral signals by direct binding of chordin to BMP-4. Piccolo S, Sasai Y, Lu B, De Robertis EM. Cell. 1996 Aug 23. 86(4):589-98. 10.1016/S0092-8674(00)80132-4 PubMed 8752213
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