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MigR1 Fli-1 GFP
(Plasmid #24668)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24668 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MigR1
  • Backbone size w/o insert (bp) 6056
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fli-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1700
  • Entrez Gene
    Fli1 (a.k.a. EWSR2, Fli-1, SIC-1, Sic1)
  • Tags / Fusion Proteins
    • IRES (C terminal on backbone)
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN-5
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fli-I cloned does not include exon 1. BamHI site (located position 468 of NM_008026) ligated with BglII.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MigR1 Fli-1 GFP was a gift from Yaacov Ben-David (Addgene plasmid # 24668 ; http://n2t.net/addgene:24668 ; RRID:Addgene_24668)

  • For your References section:

    Retroviral insertional activation of the Fli-3 locus in erythroleukemias encoding a cluster of microRNAs that convert Epo-induced differentiation to proliferation. Cui JW, Li YJ, Sarkar A, Brown J, Tan YH, Premyslova M, Michaud C, Iscove N, Wang GJ, Ben-David Y. Blood. 2007 Oct 1. 110(7):2631-40. 10.1182/blood-2006-10-053850 PubMed 17586726
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