pASTA3
(Plasmid #24657)

• Depositing Lab: Tanya Parish
• Publication: Carroll et al PLoS One. 2010 . 5(3):e9823.

Backbone

• Vector backbone: pSMT3-S
• Backbone size w/o insert (bp): 5582
• Vector type: Bacterial Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tdTomato
• Species: Synthetis gene
• Insert Size (bp): 1450

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13rev
• 3′ sequencing primer: pBluescriptKS

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Synthetic gene obtained from Jeff Cirillo
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry