2F+NFE2
(Plasmid
#24656)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24656 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF-BOS
- Backbone size w/o insert (bp) 5800
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep45 NFE2
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SpeciesM. musculus (mouse)
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Entrez GeneNfe2 (a.k.a. NF-E2, NF-E2/P45, p45, p45NFE2, p45nf-e2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EF-1a Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
2F+NFE2 was a gift from Yaacov Ben-David (Addgene plasmid # 24656 ; http://n2t.net/addgene:24656 ; RRID:Addgene_24656) -
For your References section:
Retroviral integration within the Fli-2 locus results in inactivation of the erythroid transcription factor NF-E2 in Friend erythroleukemias: evidence that NF-E2 is essential for globin expression. Lu SJ, Rowan S, Bani MR, Ben-David Y. Proc Natl Acad Sci U S A. 1994 Aug 30. 91(18):8398-402. 10.1073/pnas.91.18.8398 PubMed 8078893