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Addgene

HA SEK AL pcDNA3.1 zeo (+) - SEK1 (A-L)
(Plasmid #24653)

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Item Catalog # Description Quantity Price (USD)
Plasmid 24653 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pcDNA3.1 zeo (+)
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SEK1
  • Species
    H. sapiens (human)
  • Mutation
    Ser220Ala, Thr224Leu
  • Entrez Gene
    MAP2K4 (a.k.a. JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Leu-Val-Asp-Ala-Ile-Ala-Lys-Leu-Arg-Asp-Ala
CTTGTGGACgCTATTGCCAAGcttAGAGATGCT.

There is also A->T mutation in the following sequence LMYEER*T*VEVACY that does not alter function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA SEK AL pcDNA3.1 zeo (+) - SEK1 (A-L) was a gift from Jim Woodgett (Addgene plasmid # 24653 ; http://n2t.net/addgene:24653 ; RRID:Addgene_24653)

  • For your References section:

    Activation of stress-activated protein kinase by MEKK1 phosphorylation of its activator SEK1. Yan M, Dai T, Deak JC, Kyriakis JM, Zon LI, Woodgett JR, Templeton DJ. Nature. . 372(6508):798-800. 10.1038/372798a0 PubMed 7997270
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