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Addgene

Flag SGK3 wt - pCMV
(Plasmid #24650)

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Item Catalog # Description Quantity Price (USD)
Plasmid 24650 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    p3XFLAG-CMV-10
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SGK3
  • Species
    H. sapiens (human)
  • Entrez Gene
    SGK3 (a.k.a. CISK, SGK2, SGKL)
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag SGK3 wt - pCMV was a gift from Jim Woodgett (Addgene plasmid # 24650 ; http://n2t.net/addgene:24650 ; RRID:Addgene_24650)

  • For your References section:

    Role of the Phox homology domain and phosphorylation in activation of serum and glucocorticoid-regulated kinase-3. Tessier M, Woodgett JR. J Biol Chem. 2006 Aug 18. 281(33):23978-89. 10.1074/jbc.M604333200 PubMed 16790420
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