pCMV5 T7-Par6 WT T7 tagged
(Plasmid
#24649)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24649 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepCMV5
- Backbone size w/o insert (bp) 4600
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePar6
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SpeciesM. musculus (mouse)
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Entrez GenePard6a (a.k.a. 0710008C04Rik, 2610010A15Rik, PAR-6A, PAR6alpha, Par-6, Par6, Par6c, TAX40, Tip-40)
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Tag
/ Fusion Protein
- T7 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PCR was performed using pcDNA3-mPar6c from Tony Pawson's lab as the template. Backbone is from pCMV5B-flag-Smurf1 constructs.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV5 T7-Par6 WT T7 tagged was a gift from Jeff Wrana (Addgene plasmid # 24649 ; http://n2t.net/addgene:24649 ; RRID:Addgene_24649) -
For your References section:
Regulation of the polarity protein Par6 by TGFbeta receptors controls epithelial cell plasticity. Ozdamar B, Bose R, Barrios-Rodiles M, Wang HR, Zhang Y, Wrana JL. Science. 2005 Mar 11. 307(5715):1603-9. 10.1126/science.1105718 PubMed 15761148