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pCMV5 Flag-Par6 delta 250-295 Flag tagged
(Plasmid #24646)

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Item Catalog # Description Quantity Price (USD)
Plasmid 24646 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCMV5
  • Backbone size w/o insert (bp) 4600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Par6c
  • Alt name
    Pard6a
  • Species
    M. musculus (mouse)
  • Mutation
    Deletion of the PDZ domain of Par6c (aa 250-295)
  • Entrez Gene
    Pard6a (a.k.a. 0710008C04Rik, 2610010A15Rik, PAR-6A, PAR6alpha, Par-6, Par6, Par6c, TAX40, Tip-40)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-PA-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PCR was performed using pcDNA3-mPar6c from Tony Pawson's lab as the template. Backbone is from pCMV5B-flag-Smurf1 constructs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 Flag-Par6 delta 250-295 Flag tagged was a gift from Jeff Wrana (Addgene plasmid # 24646 ; http://n2t.net/addgene:24646 ; RRID:Addgene_24646)

  • For your References section:

    Regulation of planar cell polarity by Smurf ubiquitin ligases. Narimatsu M, Bose R, Pye M, Zhang L, Miller B, Ching P, Sakuma R, Luga V, Roncari L, Attisano L, Wrana JL. Cell. 2009 Apr 17. 137(2):295-307. 10.1016/j.cell.2009.02.025 PubMed 19379695
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