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Addgene

pGateway 3XFlag Prickle1 flag tagged
(Plasmid #24644)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 24644 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pGateway 5'CMV Trible Flag
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Prickle 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    PRICKLE1 (a.k.a. EPM1B, RILP)
  • Tag / Fusion Protein
    • 3XFLAG (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site att1 (unknown if destroyed)
  • 3′ cloning site att2 (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGateway 3XFlag Prickle1 flag tagged was a gift from Jeff Wrana (Addgene plasmid # 24644 ; http://n2t.net/addgene:24644 ; RRID:Addgene_24644)

  • For your References section:

    Regulation of planar cell polarity by Smurf ubiquitin ligases. Narimatsu M, Bose R, Pye M, Zhang L, Miller B, Ching P, Sakuma R, Luga V, Roncari L, Attisano L, Wrana JL. Cell. 2009 Apr 17. 137(2):295-307. 10.1016/j.cell.2009.02.025 PubMed 19379695
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