pKS104
(Plasmid
#24632)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24632 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneColE1?
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namefadD (M3351), atfA
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SpeciesE. coli
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
single operon, fadD (e.coli) and atfA wax ester synthase
EJ Steen et al. 2009 Nature 10.1038/nature08721
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKS104 was a gift from Jay Keasling (Addgene plasmid # 24632 ; http://n2t.net/addgene:24632 ; RRID:Addgene_24632) -
For your References section:
Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del Cardayre SB, Keasling JD.. Nature. 2010 Jan 28;463(7280):559-62. 10.1038/nature08721 PubMed 20111002
