pKS17
(Plasmid
#24628)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24628 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBBR
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepdc, adhB, atfA
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Alt namepdc, adhB, atfA, ethanol, mobilis, wax ester synthase, FAEE
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SpeciesZ. mobilis, A. baylyi ADP1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
EJ Steen et al. Nature 2009 10.1038/nature08721
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKS17 was a gift from Jay Keasling (Addgene plasmid # 24628 ; http://n2t.net/addgene:24628 ; RRID:Addgene_24628) -
For your References section:
Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del Cardayre SB, Keasling JD.. Nature. 2010 Jan 28;463(7280):559-62. 10.1038/nature08721 PubMed 20111002