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Addgene

pCMV5 MYC-Smurf2 (FF29/30A) WT
(Plasmid #24604)

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Item Catalog # Description Quantity Price (USD)
Plasmid 24604 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCMV5b
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smurf2
  • Species
    H. sapiens (human)
  • Mutation
    F29A, F30A
  • Entrez Gene
    SMURF2
  • Tag / Fusion Protein
    • MYC (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 MYC-Smurf2 (FF29/30A) WT was a gift from Jeff Wrana (Addgene plasmid # 24604 ; http://n2t.net/addgene:24604 ; RRID:Addgene_24604)

  • For your References section:

    The ubiquitin binding region of the Smurf HECT domain facilitates polyubiquitylation and binding of ubiquitylated substrates. Ogunjimi AA, Wiesner S, Briant DJ, Varelas X, Sicheri F, Forman-Kay J, Wrana JL. J Biol Chem. 2010 Feb 26. 285(9):6308-15. 10.1074/jbc.M109.044537 PubMed 20026602
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