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Addgene

pLPC-scribP305L
(Plasmid #24601)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24601 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLPC
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Scribble
  • Species
    H. sapiens (human)
  • Mutation
    P305L
  • Entrez Gene
    SCRIB (a.k.a. CRIB1, SCRB1, SCRIB1, Vartul, oSCRIB)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC-scribP305L was a gift from Senthil Muthuswamy (Addgene plasmid # 24601 ; http://n2t.net/addgene:24601 ; RRID:Addgene_24601)

  • For your References section:

    Deregulation of scribble promotes mammary tumorigenesis and reveals a role for cell polarity in carcinoma. Zhan L, Rosenberg A, Bergami KC, Yu M, Xuan Z, Jaffe AB, Allred C, Muthuswamy SK. Cell. 2008 Nov 28. 135(5):865-78. 10.1016/j.cell.2008.09.045 PubMed 19041750
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