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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24600 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLNCX2
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameScribble
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Alt nameNM_015356.3
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SpeciesH. sapiens (human)
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Entrez GeneSCRIB (a.k.a. CRIB1, SCRB1, SCRIB1, Vartul, oSCRIB)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLNCX2-Scrib was a gift from Senthil Muthuswamy (Addgene plasmid # 24600 ; http://n2t.net/addgene:24600 ; RRID:Addgene_24600) -
For your References section:
Deregulation of scribble promotes mammary tumorigenesis and reveals a role for cell polarity in carcinoma. Zhan L, Rosenberg A, Bergami KC, Yu M, Xuan Z, Jaffe AB, Allred C, Muthuswamy SK. Cell. 2008 Nov 28. 135(5):865-78. 10.1016/j.cell.2008.09.045 PubMed 19041750