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Addgene

MSCV-Flag-mPar6a.PIG
(Plasmid #24599)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Par6c
  • Species
    M. musculus (mouse)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer pLXSN 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-Flag-mPar6a.PIG was a gift from Senthil Muthuswamy (Addgene plasmid # 24599 ; http://n2t.net/addgene:24599 ; RRID:Addgene_24599)

  • For your References section:

    Par6-aPKC uncouples ErbB2 induced disruption of polarized epithelial organization from proliferation control. Aranda V, Haire T, Nolan ME, Calarco JP, Rosenberg AZ, Fawcett JP, Pawson T, Muthuswamy SK. Nat Cell Biol. 2006 Nov . 8(11):1235-45. 10.1038/ncb1485 PubMed 17060907
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