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Addgene

VCB1-CKKp
(Plasmid #24597)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24597 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ventx vector
  • Backbone manufacturer
    PMID: 12864922
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CB1-CKKp
  • Species
    Peptides
  • Tag / Fusion Protein
    • Venus (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Venus from Miyawaki (Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., and Miyawaki, A. (2002) Nat Biotechnol 20, 87-90.)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CB1 and the CKKp are the peptides PPPALPPKKR and VKLIPSWTTVILVKSMLRKRSFGNPF, respectively. All the components (Venus, CB1 and CKKp) are linked by an amino acid linker 'AS'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VCB1-CKKp was a gift from Kevin Truong (Addgene plasmid # 24597 ; http://n2t.net/addgene:24597 ; RRID:Addgene_24597)

  • For your References section:

    Rate and extent of protein localization is controlled by peptide-binding domain association kinetics and morphology. Mills E, Truong K. Protein Sci. 2009 Jun . 18(6):1252-60. 10.1002/pro.135 PubMed 19472343
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