IELMV
(Plasmid
#24596)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCfvtx
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Backbone manufacturerPMID: 12864922
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIE-LM
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SpeciesPeptides
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Tag
/ Fusion Protein
- Venus (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byVenus from Miyawaki (Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., and Miyawaki, A. (2002) Nat Biotechnol 20, 87-90).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
IE peptide: RAAITVQRYVRGYDIKEKLCYVALD
LM peptide:
RIRKKLRKNFIAVSAANRFKKI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IELMV was a gift from Kevin Truong (Addgene plasmid # 24596 ; http://n2t.net/addgene:24596 ; RRID:Addgene_24596) -
For your References section:
Engineering Ca(2+)/calmodulin-mediated modulation of protein translocation by overlapping binding and signaling peptide sequences. Mills E, Truong K. Cell Calcium. 2010 Feb 16. ():. 10.1016/j.ceca.2010.01.008 PubMed 20167369
Map uploaded by the depositor.