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Addgene

IELMV
(Plasmid #24596)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24596 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCfvtx
  • Backbone manufacturer
    PMID: 12864922

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IE-LM
  • Species
    Peptides
  • Tag / Fusion Protein
    • Venus (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Venus from Miyawaki (Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., and Miyawaki, A. (2002) Nat Biotechnol 20, 87-90).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

IE peptide: RAAITVQRYVRGYDIKEKLCYVALD

LM peptide:
RIRKKLRKNFIAVSAANRFKKI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IELMV was a gift from Kevin Truong (Addgene plasmid # 24596 ; http://n2t.net/addgene:24596 ; RRID:Addgene_24596)
  • For your References section:

    Engineering Ca(2+)/calmodulin-mediated modulation of protein translocation by overlapping binding and signaling peptide sequences. Mills E, Truong K. Cell Calcium. 2010 Feb 16. ():. 10.1016/j.ceca.2010.01.008 PubMed 20167369