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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24595 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSEAP
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Alt nameSecreted Embryonic Alkaline Phosphatase
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Alt nameALPP alkaline phosphatase
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SpeciesH. sapiens (human)
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Entrez GeneALPP (a.k.a. ALP, ALPI, IAP, PALP, PLAP, PLAP-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-pUC Forward (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CMV promoter and secreted alkaline phosphatase ORF were cloned into pBluescript
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-SEAP was a gift from Alan Cochrane (Addgene plasmid # 24595 ; http://n2t.net/addgene:24595 ; RRID:Addgene_24595)