pLAIΔmls
(Plasmid
#24594)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24594 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneN/A
- Backbone size w/o insert (bp) 7297
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHIV-1
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SpeciesHuman immunodeficiency virus 1
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MutationHuman immunodeficiency virus type 1, isolate BRU, complete genome(LAV-1). Large deletion in the pol ORF.
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Pol-Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byNIH AIDS Research and Reference Reagent Program
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A deletion with Mls1(MscI) was made in the provirus vector and the backbone was then religated to produce a replication deficient virus vector.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLAIΔmls was a gift from Alan Cochrane (Addgene plasmid # 24594 ; http://n2t.net/addgene:24594 ; RRID:Addgene_24594)
