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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24592 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLKO.1
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Growth instructionsDB3.1 or ccdB resistance strain. We do not allow E. coli harbouring this plasmid to grow to saturation (no longer than 14 hours). We prefer to grow it at 30C and 180RPM of shaking when applicable.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGateway(TM) cassette
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Insert Size (bp)1705
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Tag
/ Fusion Protein
- VA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attR1 (destroyed during cloning)
- 3′ cloning site attR2 (destroyed during cloning)
- 5′ sequencing primer GW-3' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
VA= Versatile Affinity tag, 3XFlag+6XHis+StrepIII
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLD-puro-2A-rtTA-TcVA was a gift from Jason Moffat (Addgene plasmid # 24592 ; http://n2t.net/addgene:24592 ; RRID:Addgene_24592) -
For your References section:
A lentiviral-based functional proteomics approach identifies chromatin remodelling complexes important for the induction of pluripotency. Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Punna T, Isserlin R, Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J. Mol Cell Proteomics. 2010 May;9(5):811-23. 10.1074/mcp.M000002-MCP201 PubMed 20305087