pBS_hNFATc4nuc
(Plasmid
#24586)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24586 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS.KS+
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNFATc4
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SpeciesH. sapiens (human)
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Insert Size (bp)2733
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MutationS167A, S169A, S172A, S173A, S174A, S213A, S217A, S221A
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Entrez GeneNFATC4 (a.k.a. NF-AT3, NF-ATC4, NFAT3)
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Tag
/ Fusion Protein
- EE tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS_hNFATc4nuc was a gift from Jerry Crabtree (Addgene plasmid # 24586 ; http://n2t.net/addgene:24586 ; RRID:Addgene_24586) -
For your References section:
L-type calcium channels and GSK-3 regulate the activity of NF-ATc4 in hippocampal neurons. Graef IA, Mermelstein PG, Stankunas K, Neilson JR, Deisseroth K, Tsien RW, Crabtree GR. Nature. 1999 Oct 14. 401(6754):703-8. 10.1038/44378 PubMed 10537109
