pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR
(Plasmid #24584)

• Depositing Lab: Thomas Wandless
• Publication: Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.1002/yea.1744

Backbone

• Vector backbone: pGADT7
• Backbone size w/o insert (bp): 6000
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR
• Alt name: ADH1 promoter ye Cherry
• Alt name: TEF1 (S.C.) promoter yeGFP-ecDHFR
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 3060
• Mutation: 1. Truncated version of ADH700 promoter (SbfI/SpeI) driven yeast enhanced RFP (SpeI/-). 2. promoter TEF1(S.C.) (SacII/BglII) driven yeast enhanced GFP fused to E.coli DHFR (BglII/XhoI)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: sbf1 (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The TEF1 (S.C.) promoter region has 4 base differences from the published sequences but the authors report that they do not affect transcriptional function.