pIRES2-eGFP-Ofd1-Myc-G139S
(Plasmid #24581)

• Depositing Lab: Jeremy Reiter
• Publication: Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Backbone

• Vector backbone: pIRES2-eGFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5578
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ofd1
• Species: M. musculus (mouse)
• Insert Size (bp): 3087
• Mutation: Glycine 139 changed to serine (codon GGT to AGT)
• GenBank ID: NM_177429
• Entrez Gene: Ofd1 (a.k.a. ORF2, Cxorf5, DXGgc7e)
• Tags / Fusion Proteins:
  • Myc (C terminal on insert)
  • eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: ccctaggtccccagagaca

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mutation reported in:

Clinical, molecular, and genotype-phenotype correlation studies from 25 cases of oral-facial-digital syndrome type 1: a French and Belgian collaborative study.

Thauvin-Robinet C, Cossée M, Cormier-Daire V, Van Maldergem L, Toutain A, Alembik Y, Bieth E, Layet V, Parent P, David A, Goldenberg A, Mortier G, Héron D, Sagot P, Bouvier AM, Huet F, Cusin V, Donzel A, Devys D, Teyssier JR, Faivre L.

J Med Genet. 2006 Jan;43(1):54-61.PMID: 16397067