pME.A (pDPL6)
(Plasmid
#24571)
-
Purpose(Empty Backbone)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 24571 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBR322
- Backbone size (bp) 2200
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBRrevBam (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is a derivative of pBR322. This plasmid contains a deletion from Hae II site 778 to Hae II site 2352. A polylinker has been added incorporating the Hind III and Cla I sites of pBR322. 4 bp of the EcoRI have been deleted.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pME.A (pDPL6) was a gift from David Pulleyblank (Addgene plasmid # 24571 ; http://n2t.net/addgene:24571 ; RRID:Addgene_24571) -
For your References section:
Facile transition of poly[d(TG) x d(CA)] into a left-handed helix in physiological conditions. Haniford DB, Pulleyblank DE. Nature. 1983 Apr 14. 302(5909):632-4. 10.1038/302632a0 PubMed 6835398
Map uploaded by the depositor.