WNK2 K207A
(Plasmid #24570)

• Depositing Lab: Joseph Costello
• Publication: Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 104(26):10974-9.

Backbone

• Vector backbone: pcDNA3.1/V5–His–TOPO
• Backbone size w/o insert (bp): 5523
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: WNK2`
• Species: H. sapiens (human)
• Mutation: K207A
• Entrez Gene: WNK2 (a.k.a. NY-CO-43, P/OKcl.13, PRKWNK2, SDCCAG43)
• Tags / Fusion Proteins:
  • V5 (C terminal on insert)
  • His6 (C terminal on insert)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: CMV-F, T7
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry