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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24567 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGreen H-Pelican
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Backbone manufacturerScott Barolo
- Backbone size w/o insert (bp) 10462
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Vector typereporter construct
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsFor all constructs with a Gateway RfA cassette you need DB3.1 bacterial cells for transformation or any other E. coli strain that is resistant to CcdB effects.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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Alt namepHPdestmCherry
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Speciesreporter gene mCherry
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Insert Size (bp)1722
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GenBank IDGU142866
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
AmpR in backbone; CmR in gateway RfA cassette
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHPdestmCherry was a gift from Ingrid Lohmann (Addgene plasmid # 24567 ; http://n2t.net/addgene:24567 ; RRID:Addgene_24567) -
For your References section:
Vectors for efficient and high-throughput construction of fluorescent Drosophila reporters using the PhiC31 site-specific integration system. Boy AL, Zhai Z, Habring-Müller A, Kussler-Schneider Y, Kaspar P, Lohmann I.. Genesis. 2010 Apr 22. [Epub ahead of print] 10.1002/dvg.20637 PubMed 20506180