pIRES2-eGFP-Ofd1-Myc
(Plasmid #24560)

• Depositing Lab: Jeremy Reiter
• Publication: Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Backbone

• Vector backbone: pIRES2-eGFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5578
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ofd1
• Species: M. musculus (mouse)
• Insert Size (bp): 3087
• GenBank ID: NM_177429
• Entrez Gene: Ofd1 (a.k.a. ORF2, Cxorf5, DXGgc7e)
• Tags / Fusion Proteins:
  • Myc (C terminal on insert)
  • eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: ccctaggtccccagagaca

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry