pCMV/SB10ΔDDE
(Plasmid #24552)

• Depositing Lab: Perry Hackett
• Publication: Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Backbone

• Vector backbone: pCMVbeta
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3712
• Modifications to backbone: pCMV/SB10 (Addgene plasmid # 24551) was cut with MscI to remove 322 bp of the transposase coding region which deleted much of the catalytic DDE domain and disrupted the reading frame by introducing a premature translational termination codon.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sleeping Beauty delta DDE
• Alt name: SBdDDE
• Alt name: SB10dDDE
• Insert Size (bp): 698
• Mutation: removes 322 bp of the transposase-coding region
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (destroyed during cloning)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: M13 Forward
• 3′ sequencing primer: M13 Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry