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Addgene

pCMV/SB10
(Plasmid #24551)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24551 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMVbeta
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3712
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sleeping Beauty
  • Alt name
    SB
  • Alt name
    SB10
  • Insert Size (bp)
    1020

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (destroyed during cloning)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer M13 Forward
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV/SB10 was a gift from Perry Hackett (Addgene plasmid # 24551 ; http://n2t.net/addgene:24551 ; RRID:Addgene_24551)

  • For your References section:

    Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Ivics Z, Hackett PB, Plasterk RH, Izsvak Z. Cell. 1997 Nov 14. 91(4):501-10. 10.1016/S0092-8674(00)80436-5 PubMed 9390559
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