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pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR
(Plasmid #24549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGADT7
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pADH1 yeCherry::YAP1 yeGFP-DHFR
  • Alt name
    ADH1 promoter ye Cherry
  • Alt name
    YAP1 IRES yeGFP-ecDHFR
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2830
  • Mutation
    1. Truncated version of ADH700 promoter (SbfI/SpeI) driven yeast enhanced RFP (SpeI/-). 2. YAP1 IRES (SacII/BglII) driven yeast enhanced GFP fused to E.coli DHFR (BglII/XhoI)
  • Entrez Gene
    YAP1 (a.k.a. YML007W, PAR1, PDR4, SNQ3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site sbf1 (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR was a gift from Thomas Wandless (Addgene plasmid # 24549 ; http://n2t.net/addgene:24549 ; RRID:Addgene_24549)

  • For your References section:

    Dicistronic regulation of fluorescent proteins in the budding yeast Saccharomyces cerevisiae. Edwards SR, Wandless TJ. Yeast. 2010 Apr;27(4):229-36. doi: 10.1002/yea.1744 10.1002/yea.1744 PubMed 20017217
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