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Addgene

pN3-Sp1FL
(Plasmid #24543)

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Item Catalog # Description Quantity Price (USD)
Plasmid 24543 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pN3
  • Backbone manufacturer
    Guntram Suske, Addgene plasmid 24544
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sp1FL
  • Alt name
    transcription factor Sp1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4200
  • Entrez Gene
    SP1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer AATCAACGGGACTTTCC, CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pN3-Control vector also available from Addgene

2 nucleotide mismatches with author sequence detected in the most 3´-UTR. They do not affect the coding sequence of Sp1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pN3-Sp1FL was a gift from Guntram Suske (Addgene plasmid # 24543 ; http://n2t.net/addgene:24543 ; RRID:Addgene_24543)
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