pN3-Sp3FL
(Plasmid #24541)

• Depositing Lab: Guntram Suske
• Publication: Sapetschnig et al J Biol Chem. 2004 Oct 1. 279(40):42095-105.

Backbone

• Vector backbone: pN3
• Backbone manufacturer: Guntram Suske, Addgene plasmid 24544
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: transcription factor Sp3
• Species: H. sapiens (human)
• Insert Size (bp): 2740
• Entrez Gene: SP3 (a.k.a. SPR2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: AATCAACGGGACTTTCC, CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Two nucleotide mismatches with author sequence detected (one adjacent to the EcoRI cloning site and the second in the middle of the 5´-UTR). They do not affect the coding sequence of Sp3.