pBabe-Ypet-PBD
(Plasmid #24533)

• Depositing Lab: Klaus Hahn
• Publication: Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Backbone

• Vector backbone: pBabe-sin-tet-CMV-puro
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PAK binding domain
• Species: H. sapiens (human)
• Insert Size (bp): 300
• Entrez Gene: PAK1 (a.k.a. IDDMSSD, PAKalpha, alpha-PAK, p65-PAK)
• Tag / Fusion Protein:
  • Ypet (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: HpaI (destroyed during cloning)
• 5′ sequencing primer: pBabe 5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Protocols for production and use of Hahn lab biosensors and photoactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments!

Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html