pHAGE-EF1alphaL-hAAT-W
(Plasmid #24527)

• Purpose: Lentiviral overexpression
• Depositing Lab: Darrell Kotton
• Publication: Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Backbone

• Vector backbone: pHAGE
• Backbone manufacturer: Richard Mulligan
• Backbone size w/o insert (bp): 4874
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: alpha-1 antitrypsin
• Species: H. sapiens (human)
• Insert Size (bp): 3411
• Entrez Gene: SERPINA1 (a.k.a. A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, nNIF)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: EF-1a Forward

Resource Information

• Supplemental Documents:
  • pHAGE-EFalpha1L-hAAT-W
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry