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PurposeLentiviral overexpression
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 24527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHAGE
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Backbone manufacturerRichard Mulligan
- Backbone size w/o insert (bp) 4874
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namealpha-1 antitrypsin
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SpeciesH. sapiens (human)
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Insert Size (bp)3411
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Entrez GeneSERPINA1 (a.k.a. A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, nNIF)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EF-1a Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE-EF1alphaL-hAAT-W was a gift from Darrell Kotton (Addgene plasmid # 24527 ; http://n2t.net/addgene:24527 ; RRID:Addgene_24527) -
For your References section:
Amelioration of emphysema in mice through lentiviral transduction of long-lived pulmonary alveolar macrophages. Wilson AA, Murphy GJ, Hamakawa H, Kwok LW, Srinivasan S, Hovav AH, Mulligan RC, Amar S, Suki B, Kotton DN. J Clin Invest. 2010 Jan . 120(1):379-89. 10.1172/JCI36666 PubMed 20038801
Map uploaded by the depositor.
