pAdEasy-EGFP-CLASP2 340-1362 9xS/A
(Plasmid #24523)

• Depositing Lab: Torsten Wittmann
• Publication: Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908.

Backbone

• Vector backbone: pAdEasy-1
• Backbone manufacturer: Stratagene, ATCC
• Backbone size w/o insert (bp): 33414
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CLASP2 (340-1362) 9xS/A
• Alt name: CLASP2
• Species: H. sapiens (human)
• Insert Size (bp): 3800
• Mutation: Nonphosphorylatable CLASP2 deletion mutant. Mutation of serines in GSK3β motif between S594 to S614 plus S568/572/576A.
• Entrez Gene: CLASP2
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F, EGFP-C

Resource Information

• Supplemental Documents:
  • CLASP2 Reference sequence.
• A portion of this plasmid was derived from a plasmid made by: CLASP2 cDNA was a gift from Anna Akhmanova

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for.

Due to difficulty in sequencing this plasmid, the EGFP and C-terminus of CLASP2 has not been sequence verified.

EGFP-CLASP2 340-1362 9xS/A was cloned into pShuttle-CMV between KpnI and NotI then recombined by homologous recombination into pAdEasy-1.

AdEasy is a registered trademark of the Johns Hopkins University.