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Addgene

pCMV-ECFPEYFP
(Plasmid #24520)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24520 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFPC1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ECFP, EYFP
  • Alt name
    CFP,YFP
  • Species
    Vibrio fischeri
  • Insert Size (bp)
    757
  • GenBank ID
    P21578
  • Tags / Fusion Proteins
    • ECFP (C terminal on backbone)
    • EYFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bsp E1 (unknown if destroyed)
  • 3′ cloning site Bgl II (unknown if destroyed)
  • 5′ sequencing primer AAGTCCGGAATGGTGAGCAAGGGCGAGGA
  • 3′ sequencing primer TCAAGATCTCTTGTACAGCTCGTCCATGCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-ECFPEYFP was a gift from Liusheng He & Jehonathan Pinthus (Addgene plasmid # 24520 ; http://n2t.net/addgene:24520 ; RRID:Addgene_24520)

  • For your References section:

    A flow cytometric method to detect protein-protein interaction in living cells by directly visualizing donor fluorophore quenching during CFP-->YFP fluorescence resonance energy transfer (FRET). He L, Olson DP, Wu X, Karpova TS, McNally JG, Lipsky PE. Cytometry A. 2003 Oct . 55(2):71-85. 10.1002/cyto.a.10073 PubMed 14505312
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