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Addgene

pENTR-Site2
(Plasmid #24513)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24513 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2538
  • Vector type
    Mammalian Expression, Mouse Targeting, Cre/Lox
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Split RFP-GFP chimera
  • Species
    Aequorea victoria and Discosoma sp. reef coral
  • Insert Size (bp)
    2984

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attL1 (not destroyed)
  • 3′ cloning site attL2 (not destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-Site2 was a gift from Kazuo Kinoshita (Addgene plasmid # 24513 ; http://n2t.net/addgene:24513 ; RRID:Addgene_24513)

  • For your References section:

    Chromosomal manipulation by site-specific recombinases and fluorescent protein-based vectors. Uemura M, Niwa Y, Kakazu N, Adachi N, Kinoshita K. PLoS One. 2010 . 5(3):e9846. 10.1371/journal.pone.0009846 PubMed 20352097
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