NK-1 GFP
(Plasmid
#24508)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24508 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET17b
- Backbone size w/o insert (bp) 3300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDrosophila NCD, human kinesin
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Alt nameNCD fused to human kinesin
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SpeciesH. sapiens (human), D. melanogaster (fly)
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MutationNK-1 is a chimera where the entire superfamily-conserved catalytic domain (aa 348-667) of NCD was substituted for the equivalent domain of inesin (aa 8-322). GFP is mutant (S65T)
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Tags
/ Fusion Proteins
- GFP (C terminal on insert)
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer GFP-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NK-1 GFP was a gift from Ron Vale (Addgene plasmid # 24508 ; http://n2t.net/addgene:24508 ; RRID:Addgene_24508) -
For your References section:
The directional preference of kinesin motors is specified by an element outside of the motor catalytic domain. Case RB, Pierce DW, Hom-Booher N, Hart CL, Vale RD. Cell. 1997 Sep 5. 90(5):959-66. 10.1016/S0092-8674(00)80360-8 PubMed 9298907