pFLAG UBR1 SBX
(Plasmid #24506)

• Depositing Lab: Alexander Varshavsky
• Publication: Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-28.

Backbone

• Vector backbone: Yeplac 181
• Backbone size w/o insert (bp): 5741
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UBR1
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: UBR1 (a.k.a. YGR184C, PTR1)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: yADH1-F
• 3′ sequencing primer: M13pUC-Rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SBX stands for SalI, BamHI, XhoI. SalI is 15 bp before ATG codon of UBR1ORF. BamHI and XhoI are right after the stop codon of the UBR1ORF.
The plasmid expresses UBR1 which is N-terminally Flag tagged and has "clean" C-terminus.
Note that it does not have NcoI site upstream of ATG codon. Active in degrading N-end rule substrates.