pRBUBR1empty
(Plasmid
#24505)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24505 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRBUBR1
- Backbone size (bp) 5000
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SphI (unknown if destroyed)
- 3′ cloning site SphI (unknown if destroyed)
- 5′ sequencing primer M13_puc_reverse
- 3′ sequencing primer M13_puc_F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid created by digesting pRBUBR1 with Sph I and ligate the vector backbone together to remove UBR1ha gene. ADH promoter is still present.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRBUBR1empty was a gift from Alexander Varshavsky (Addgene plasmid # 24505 ; http://n2t.net/addgene:24505 ; RRID:Addgene_24505) -
For your References section:
Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end rule pathway. Xia Z, Webster A, Du F, Piatkov K, Ghislain M, Varshavsky A. J Biol Chem. 2008 Aug 29. 283(35):24011-28. 10.1074/jbc.M802583200 PubMed 18566452