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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24500 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA1
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Tetracycline, 100 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10/P3
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Growth instructionsPlasmid contains SupF. Grow in TOP10/P3 cells.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGq alpha
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Alt nameG protein alpha subunit
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Alt nameGnaq
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SpeciesM. musculus (mouse)
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MutationGq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY)
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Entrez GeneGnaq (a.k.a. 1110005L02Rik, 6230401I02Rik, Dsk1, Dsk10, Galphaq, Gq, GqI)
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Tag
/ Fusion Protein
- HA (see comments)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site NsiI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY). Works the same as qi5 but (for unknown reasons) has a slightly lower basal PLC activity. This can increase the signal-to-noise ratio.
Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
qo5 was a gift from Bruce Conklin (Addgene plasmid # 24500 ; http://n2t.net/addgene:24500 ; RRID:Addgene_24500) -
For your References section:
Substitution of three amino acids switches receptor specificity of Gq alpha to that of Gi alpha. Conklin BR, Farfel Z, Lustig KD, Julius D, Bourne HR. Nature. 1993 May 20. 363(6426):274-6. 10.1038/363274a0 PubMed 8387644