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pcDNA3.1mLCAD_8KR_FLAG
(Plasmid #24485)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24485 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Long chain acyl-CoA dehydrogenase
  • Alt name
    LCAD
  • Alt name
    acadl
  • Alt name
    LCAD-8KR
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Mutation
    Lys42, 156, 189, 240, 254, 318, 322, 358Arg (8KR)
  • GenBank ID
    BC027412
  • Entrez Gene
    Acadl (a.k.a. AA960361, AU018452, C79855, LCAD)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Low acetylation signal, no detectable enzymatic activity

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1mLCAD_8KR_FLAG was a gift from Eric Verdin (Addgene plasmid # 24485 ; http://n2t.net/addgene:24485 ; RRID:Addgene_24485)

  • For your References section:

    SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation. Hirschey MD, Shimazu T, Goetzman E, Jing E, Schwer B, Lombard DB, Grueter CA, Harris C, Biddinger S, Ilkayeva OR, Stevens RD, Li Y, Saha AK, Ruderman NB, Bain JR, Newgard CB, Farese RV, Alt FW, Kahn CR, Verdin E. Nature. 2010 Mar 4. 464(7285):121-5. 10.1038/nature08778 PubMed 20203611
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