Tet-inducible- FLAG-Core R (pMO30)
(Plasmid
#24479)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 24479 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepTet Splice/pEV150-HindIII
- Backbone size w/o insert (bp) 5178
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCore R
-
SpeciesHepatitis C virus
-
Insert Size (bp)600
-
Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Tet-inducible- FLAG-Core R (pMO30) was a gift from Melanie Ott (Addgene plasmid # 24479 ; http://n2t.net/addgene:24479 ; RRID:Addgene_24479) -
For your References section:
Targeting of hepatitis C virus core protein to mitochondria through a novel C-terminal localization motif. Schwer B, Ren S, Pietschmann T, Kartenbeck J, Kaehlcke K, Bartenschlager R, Yen TS, Ott M. J Virol. 2004 Aug . 78(15):7958-68. 10.1128/JVI.78.15.7958-7968.2004 PubMed 15254168
Map uploaded by the depositor.