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Addgene

Tet-inducible- FLAG-Core R (pMO30)
(Plasmid #24479)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24479 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTet Splice/pEV150-HindIII
  • Backbone size w/o insert (bp) 5178
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Core R
  • Species
    Hepatitis C virus
  • Insert Size (bp)
    600
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tet-inducible- FLAG-Core R (pMO30) was a gift from Melanie Ott (Addgene plasmid # 24479 ; http://n2t.net/addgene:24479 ; RRID:Addgene_24479)
  • For your References section:

    Targeting of hepatitis C virus core protein to mitochondria through a novel C-terminal localization motif. Schwer B, Ren S, Pietschmann T, Kartenbeck J, Kaehlcke K, Bartenschlager R, Yen TS, Ott M. J Virol. 2004 Aug . 78(15):7958-68. 10.1128/JVI.78.15.7958-7968.2004 PubMed 15254168